#Detectx corticosterone enzyme immunoassay kit pdf software#
The concentration of the E1S in the sample is calculated, after making suitable correction for the dilution of the sample, using software available with most plate readers. After a short incubation, the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring 450 nm wavelength. The substrate reacts with the bound E1S-peroxidase conjugate. After a 2 hour incubation the plate is washed and substrate is added. The binding reaction is initiated by the addition of a polyclonal antibody to E1S to each well. An E1S-peroxidase conjugate is added to the standards and samples in the wells. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An E1S standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Please read the complete kit insert before performing this assay. The kit will quantitatively measure E1S present in diluted buffer samples and tissue culture media samples. 1985 579.ģ K038-H WEB 210301 ASSAY PRINCIPLE The DetectX® Estrone-3-Sulfate (E1S) Immunoassay Kit uses a specifically generated antibody to measure E1S in a variety of matrices, including serum, plasma, urine and fecal samples. Cryptorchidism in the horse-genetic implications, in Proceedings. Liepold HW, DeBowes RM, Bennett S, et al. Comparison of hormonal methods for diagnosis of cryptorchidism in horses. Oestrogen receptors and metabolism of oestrone sulphate in human mammary carcinoma. Wilking N, Carlstrom WK, Gustafsson SA, et al. Steroid sulfatase activities in human breast tumors. Estimation of oestrone sulphate, oestradiol-17 and oestrone in peripheral plasma: concentrations during the menstrual cycle and in men. A specific radioimmunoassay for estrone sulfate in plasma and urine without hydrolysis. Wright K, CollinsDC, Musey P1, Preedy JRK. The metabolism of estrone sulfate in normal males. Estrone Sulfate: Production Rate and Metabolism in Man. Hoffmann, B, Wagner, WC, Hoxon, JE, Bahr, J., Observations concnerning the functional status of the corpus luteum and the placenta around parturition. Several investigators have suggested measuring testosterone and estrone sulfate serum levels as reliable diagnostic aids for the condition8,9.ġ. Thus, the correct laboratory diagnosis of this condition is very important, especially when exploratory abdominal surgery is considered for the removal of retained testes. Bilateral cryptorchids, must be differentiated from geldings who exhibit stallion like behavior. Bilaterally cryptorchid stallions do not produce viable spermatozoa but often exhibit normal secondary sexual characteristics such as libido, because of testosterone production by the interstitial cells of the retained testes. OĮstrone-3-Sulfate, E1S H O HH HO S O OH Cryptorchidism is a condition in which one or both testicles fail to descend into the scrotum, and it is considered to be a prevalent defect in horses8,9. Consequently, estrone sulfate provides a continuous supply of estrogens to hormone-responsive tumors. Breast tumors contain sulfatase activity6 and can convert estrone sulfate into estradiol7. In postmenopausal women with breast cancer, estrone sulfate concentrations in plasma have the same order of magnitude. Estrone sulfate is quantitatively the most important circulating estrogen. Estrone sulfate is only slowly cleared from plasma, thus its concentration does not fluctuate markedly during the day4,5. Estrone sulfate, present in plasma in a higher concentration than either unconjugated estrone or estradiol in nonpregnant women and normal men, appears to originate almost entirely from a conjugation of estrone and converted estradiolin nonglandular tissues3. Production rates of E1S are high in both males and females, with males producing 77 µg/day, and in women in early follicular phase, 95 µg/day and in early luteal phase, 182 µg/day2. 11-13Ģ EXPECT ASSAY ARTISTRY K038-H WEB 210301 BACKGROUNDĮstrone-3-sulfate, C18H22O5S, (1, 3, 5(10)-Estratrien-3-ol-17-one sulfate, E1S) is synthesized in the fetal or cotyledonary portion of the placentome1. Validation Data Sensitivity, Linearity, etc. Please read this insert completely prior to using the product. Detectx® Estrone-3-Sulfate (E1S) Enzyme Immunoassay KitĭetectX® Estrone-3- Sulfate (E1S) Enzyme Immunoassay Kitġ Plate Kit Catalog Number K038-H1 5 Plate Kit Catalog Number K038-H5ĭried Fecal Extracts, Urine, Serum/Plasma, and Tissue Culture Media
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